|Call Toll Free (888) 950-2888
Monday-Friday 8:00am-6:00pm PST International: (909) 393 9488
a division of iScope Corp, USA
During that historic period known as the Renaissance, after the "dark" Middle Ages, there occurred the
inventions of printing, gunpowder and the mariner's compass, followed by the discovery of America.
Equally remarkable was the invention of the light microscope: an instrument that enables the human
eye, by means of a lens or combinations of lenses, to observe enlarged images of tiny objects. It
made visible the fascinating details of worlds within worlds.
Invention of Glass Lenses
Long before, in the hazy unrecorded past, someone picked up a piece of transparent crystal thicker in
the middle than at the edges, looked through it, and discovered that it made things look larger.
Someone also found that such a crystal would focus the sun's rays and set fire to a piece of
parchment or cloth. Magnifiers and "burning glasses" or "magnifying glasses" are mentioned in the
writings of Seneca and Pliny the Elder, Roman philosophers during the first century A. D., but
apparently they were not used much until the invention of spectacles, toward the end of the 13th
century. They were named lenses because they are shaped like the seeds of a lentil.
The earliest simple microscope was merely a tube with a plate for the object at one end and, at the
other, a lens which gave a magnification less than ten diameters -- ten times the actual size. These
excited general wonder when used to view fleas or tiny creeping things and so were dubbed "flea
Birth of the Light Microscope
About 1590, two Dutch spectacle makers, Zaccharias Janssen and his son Hans, while experimenting
with several lenses in a tube, discovered that nearby objects appeared greatly enlarged. That was the
forerunner of the compound microscope and of the telescope. In 1609, Galileo, father of modern
physics and astronomy, heard of these early experiments, worked out the principles of lenses, and
made a much better instrument with a focusing device. The father of microscopy, Anton van
Leeuwenhoek of Holland (1632-1723), started as an apprentice in a dry goods store where magnifying
glasses were used to count the threads in cloth. He taught himself new methods for grinding and
polishing tiny lenses of great curvature which gave magnifications up to 270 diameters, the finest
known at that time. These led to the building of his microscopes and the biological discoveries for
which he is famous. He was the first to see and describe bacteria, yeast plants, the teeming life in a
drop of water, and the circulation of blood corpuscles in capillaries. During a long life he used his
lenses to make pioneer studies on an extraordinary variety of things, both living and non living, and
reported his findings in over a hundred letters to the Royal Society of England and the French Academy.
Robert Hooke, the English father of microscopy, re-confirmed Anton van Leeuwenhoek's discoveries of
the existence of tiny living organisms in a drop of water. Hooke made a copy of Leeuwenhoek's light
microscope and then improved upon his design.
Charles A. Spencer
Later, few major improvements were made until the middle of the 19th century. Then several European
countries began to manufacture fine optical equipment but none finer than the marvelous instruments
built by the American, Charles A. Spencer, and the industry he founded. Present day instruments,
changed but little, give magnifications up to 1250 diameters with ordinary light and up to 5000 with
Beyond the Light Microscope
A light microscope, even one with perfect lenses and perfect illumination, simply cannot be used to
distinguish objects that are smaller than half the wavelength of light. White light has an average
wavelength of 0.55 micrometers, half of which is 0.275 micrometers. (One micrometer is a thousandth
of a millimeter, and there are about 25,000 micrometers to an inch. Micrometers are also called
microns.) Any two lines that are closer together than 0.275 micrometers will be seen as a single line,
and any object with a diameter smaller than 0.275 micrometers will be invisible or, at best, show up as
a blur. To see tiny particles under a microscope, scientists must bypass light altogether and use a
different sort of "illumination," one with a shorter wavelength.
The introduction of the electron microscope in the 1930's filled the bill. Co-invented by Germans, Max
Knott and Ernst Ruska in 1931, Ernst Ruska was awarded half of the Nobel Prize for Physics in 1986
for his invention (The other half of the Nobel Prize was divided between Heinrich Rohrer and Gerd
Binnig for the STM). In this kind of microscope, electrons are speeded up in a vacuum until their
wavelength is extremely short, only one hundred-thousandth that of white light. Beams of these
fast-moving electrons are focused on a cell sample and are absorbed or scattered by the cell's parts
so as to form an image on an electron-sensitive photographic plate.
Light Microscope Electron Microscope