Open Box 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination
Key Features
- 40X to 600X magnification range using a quintuple lens turret for five unique settings.
- An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
- The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
- The infinity-corrected optical system ensures high-quality, high-resolution images.
- Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
- The trinocular photo port provides a dedicated interface for mounting a camera.
The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.
The Viewing Head
The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.
Optics
The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.
The Stage
The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.
Koehler Transmitted Illumination
The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.
Epi-fluorescence Illumination
A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.
Fluorescence
| Filters | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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| Mercury HBO Excitation | ||||||||||||||||||
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Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.
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Specifications
Technical drawings with measurements (in mm)
| Optical System | Infinity-corrected | ||||||||
| Reference Focal Length | 220mm | ||||||||
| Head | Trinocular, 45° incline | ||||||||
| Interpupillary Adjustment | Siedentopf compensation-free gemel, 50mm-76mm | ||||||||
| Ocular Diameter | 30mm | ||||||||
| Ocular Diopters | left ocular | ||||||||
| Eyepieces | 10X | ||||||||
| Photo-port | CX-type height-adjustable, 23mm tube | ||||||||
| Objective Lenses | Infinity-corrected | ||||||||
| Objective Turret | quintuple | ||||||||
| Focusing System |
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| Stage | fixed-height, mechanical X-Y translation | ||||||||
| Stage Dimensions | 160mm x 250mm stage extension: 70mm x 180mm |
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| Stage X-Y Travel Range | 120mm x 80mm | ||||||||
| Specimen Holders | Terasaki plate, Petri dish | ||||||||
| Episcopic Illumination (Fluorescence) | 100W mercury HBO arc-lamp, centerable | ||||||||
| Transmitted Illumination | 6V 30W halogen variable intensity centerable lamp |
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| Condenser | NA 0.3, centerable | ||||||||
| Condenser Working Distance | 72mm maximum | ||||||||
| Phase Contrast Annuli | 4X/10X and 20X/40X | ||||||||
| Power | 90-240VAC 50/60Hz wide-band | ||||||||
Objective Lenses
| Magnification | Corrections | NA | Working Distance | Cover-glass Thickness |
|---|---|---|---|---|
| 4X | plan achromatic | 0.13 | 10.4mm | 1.5mm |
| 10X | plan achromatic | 0.25 | 7.30mm | 1.5mm |
| 20X | plan achromatic | 0.40 | 6.79mm | 1.5mm |
| 40X | plan achromatic | 0.60 | 3.08mm | 1.5mm |
| 60X | plan achromatic | 0.70 | 1.71mm | 1.5mm |
| 10X | phase-ring | 0.25 | 7.30mm | 1.5mm |
| 20X | phase-ring | 0.40 | 6.80mm | 1.5mm |
| 40X | phase-ring | 0.60 | 3.08mm | 1.5mm |
Packing List:
AmScope products are warranted against manufacturer’s defects in material and workmanship from the original date of purchase, for the following years:
• Five Year Warranty for Microscopes: All our microscopes come with a five (5) year factory warranty against manufacturing defects, covering labor and parts.
• Ten Year Warranty for Euromex Microscopes: All our Euromex microscopes come with a ten (10) year factory warranty against manufacturing defects, covering labor and parts.
• One Year Warranty for Telescopes: All our telescopes come with a one (1) year warranty, except when specified in the description.
• One Year Warranty for Accessories: All our microscope accessories (USB cameras, illuminators, illumination, dust covers, and etc.) come with a one (1) year warranty, except when specified in the description.
• One Year Warranty for Items labeled as “Product Ships From Manufacturer”: All products that are shipped directly from the manufacturer come with a one (1) year warranty, except when specified in the description.
Warranty does not apply for consumable accessories and parts such as bulbs, batteries, fuses, cords, optical components or any add-on accessories such as mechanical specimen holders that are not built into the microscope stage as an integral part of the original manufacture, etc. Warranty does not cover microscopes, lenses cameras or other accessories that have become inoperable due to dirt or damage due to misuse or lack of maintenance.
Note: Buyers are responsible for return shipping and handling cost for warranty services. Our warranty covers parts and labor only.
The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.
The Viewing Head
The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.
Optics
The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.
The Stage
The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.
Koehler Transmitted Illumination
The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.
Epi-fluorescence Illumination
A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.
Fluorescence
| Filters | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
||||||||||||||||||
| Mercury HBO Excitation | ||||||||||||||||||
|
Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.
|
Specifications
Technical drawings with measurements (in mm)
| Optical System | Infinity-corrected | ||||||||
| Reference Focal Length | 220mm | ||||||||
| Head | Trinocular, 45° incline | ||||||||
| Interpupillary Adjustment | Siedentopf compensation-free gemel, 50mm-76mm | ||||||||
| Ocular Diameter | 30mm | ||||||||
| Ocular Diopters | left ocular | ||||||||
| Eyepieces | 10X | ||||||||
| Photo-port | CX-type height-adjustable, 23mm tube | ||||||||
| Objective Lenses | Infinity-corrected | ||||||||
| Objective Turret | quintuple | ||||||||
| Focusing System |
|
||||||||
| Stage | fixed-height, mechanical X-Y translation | ||||||||
| Stage Dimensions | 160mm x 250mm stage extension: 70mm x 180mm |
||||||||
| Stage X-Y Travel Range | 120mm x 80mm | ||||||||
| Specimen Holders | Terasaki plate, Petri dish | ||||||||
| Episcopic Illumination (Fluorescence) | 100W mercury HBO arc-lamp, centerable | ||||||||
| Transmitted Illumination | 6V 30W halogen variable intensity centerable lamp |
||||||||
| Condenser | NA 0.3, centerable | ||||||||
| Condenser Working Distance | 72mm maximum | ||||||||
| Phase Contrast Annuli | 4X/10X and 20X/40X | ||||||||
| Power | 90-240VAC 50/60Hz wide-band | ||||||||
Objective Lenses
| Magnification | Corrections | NA | Working Distance | Cover-glass Thickness |
|---|---|---|---|---|
| 4X | plan achromatic | 0.13 | 10.4mm | 1.5mm |
| 10X | plan achromatic | 0.25 | 7.30mm | 1.5mm |
| 20X | plan achromatic | 0.40 | 6.79mm | 1.5mm |
| 40X | plan achromatic | 0.60 | 3.08mm | 1.5mm |
| 60X | plan achromatic | 0.70 | 1.71mm | 1.5mm |
| 10X | phase-ring | 0.25 | 7.30mm | 1.5mm |
| 20X | phase-ring | 0.40 | 6.80mm | 1.5mm |
| 40X | phase-ring | 0.60 | 3.08mm | 1.5mm |