40X-400X Trinocular Inverted Biological Microscope with Phase-contrast

SKU: IN300T-FL

Availability: In Stock

Regular price $6,728.99 $4,499.99
>> How to Buy A Microscope

SELECTING A COMPOUND MICROSCOPE is as easy as 1-2-3...

Select the type of microscope you need or your industry

Select by type of microscope - Our Compound Microscope Assortment has been divided into major categories to make your shopping easy.

These categories include:

Brightfield, Darkfield, Phase-Contrast, Inverted and others

Choose a business application or industry to find the right compound microscope. Major industries or applicatins include:

Veterinary, Laboratory Use, Teaching, Metallurgical, Gemology and many others

Select a Magnification

AmScope offers an industry-leading selection of magnifications. All products are in-stock and usually ship the same day ordered

Select a Camera

We offer a wide variety of Cameras for every application - from student to professional use:

Select a type of camera - Eyepiece, USB 2.0 or USB 3.0 - to name the most popular choices

Choose the Megapixels of the camera - the higher the megapixel count on a camera sensor the greater the amount of detail that can be captured in an image

The Higher the Magnification - The More Detail You'll See

Magnification 40x
Magnification 1000x
Magnification 2500x

Magnification is the ability of your microscope to make small objects seem larger. As the magnification increases the area being viewed will be decrease is size but the detail of the area being viewed will improve.

Amscope microscopes come with multiple magnifications to aid you in finding the proper magnification for viewing each specimen.

Camera Connection Types

When comaring 2.0 and 3.0 there are few major differences. First the transfer rates. USB 2.0 offers transfer rates of 480 Mbps and USB 3.0 offers transfer rates of 4.8 Gbps - that's 10 times faster.

Camera Resolution

The most important factor in ensuring successful microscopic imaging is choosing the appropriate optics and camera for your application that gives you more data and higher image quality. When selecting a microscope camera, it is best to base your decision on the most important requirements for your microscopic observation.

Key Features

  • 40X to 400X magnification range using a quadruple lens turret for four unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.

The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

Transmitted Illumination

The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Phase Contrast

The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

Epi-fluorescence Illumination

For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

Fluorescence

Filters
Filter Set Excitation Filter Emission Filter
B 450-490nm 520nm
G 510-560nm 590nm
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Specifications

Optical System Infinity-corrected
Head Trinocular, 45° incline
Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
Ocular Diameter 30mm
Ocular Diopters right ocular
Eyepieces 10X
Photo-port height-adjustable 23mm tube
Objective Lenses Infinity-corrected
Objective Turret Quadruple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Stage fixed-height, mechanical X-Y translation
Stage Dimensions 172mm x 242mm
Stage X-Y Travel Range 75mm x 40mm
Specimen Holders Terasaki plate, Petri dish
Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
Transmitted Illumination 12V 30W halogen
variable intensity
Condenser NA 0.3, iris diaphragm, filter slot
Power 110VAC 60Hz

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X plan fluor 0.15 28mm 1.5mm
10X plan fluor, phase-contrast 0.30 3mm 1.5mm
20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
40X plan fluor 0.60 2.23mm 1.5mm

Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • attribute microscope
    camera connection type N/A
    compatibility HDMI & PC
    field view Extreme Widefield
    head style Microscopes-Trinocular
    illumination style Incident & Transmitted
    key features
  • 40X to 400X magnification range using a quadruple lens turret for four unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • light type Halogen, Fluorescent
    magnification power config 40X-400X
    magnifying power Microscope-40X to 400X
    Manufacturer AmScope
    microscope application Clinic, Veterinary, Laboratory
    microscope color White
    microscope condenser type Brightfield
    microscope eyepiece power 10X
    microscope eyepiece size 30mm
    microscope field view Extreme Widefield
    microscope head style Trinocular
    microscope light power 30W, 100W
    microscope light shape Built-in
    microscope light type Halogen, Fluorescent
    microscope magnifying power 40X to 400X
    microscope num of bulbs 1
    microscope objective power 4X, 10X, 20X, 40X
    microscope objective size 20mm
    microscope optics Infinity, Plan
    microscope power supply 110V
    microscope specialty EPI-Fluorescence, Inverted
    microscope stage div 1.00mm
    ms illuminatiostyle Incident & Transmitted
    optics Infinity, Semi-Apochromatic, Plan
    optics m Infinity, Semi-Apochromatic, Plan
    pixel N/A
    product video ids er7q4CfL26c
    prop 65 Prop 65 Warning
    specialty Microscope-EPI-Fluorescence, Microscope-Inverted
    upc 13964566383
    vendor AmScope

    The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • People Also Viewed

    Recently Viewed