EPI-Fluorescence Microscopes

A fluorescence microscope is a conventional compound microscope equipped with a high-intensity light source that emits light in a broad spectrum from visible through ultraviolet. Most utilize incident illumination to illuminate the sample from above - in this way the objective lens is used as both the illumination condenser and the fluorescent light collector. The arrangement of optical components that permits illumination from above the specimen is termed epi-fluorescence illumination.