AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-400X Magnification with 1.4MP Cooled Color CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-400X Magnification with 1.4MP Cooled Monochrome CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-400X Magnification with 6MP Cooled Color CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-600X Magnification with 1.4MP Cooled Color CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-600X Magnification with 1.4MP Cooled Monochrome CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-600X Magnification with 6MP Cooled Color CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-800X Magnification with 1.4MP Cooled Color CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-800X Magnification with 1.4MP Cooled Monochrome CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-800X Magnification with 6MP Cooled Color CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-1000X Magnification with 1.4MP Cooled Color CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-1000X Magnification with 1.4MP Cooled Monochrome CCD Camera AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-1000X Magnification with 6MP Cooled Color CCD Camera

SKU: IN300T-FL-MF143C IN300T-FL-MF143 IN300T-FL-MF603 IN300TA-FL-MF143C IN300TA-FL-MF143 IN300TA-FL-MF603 IN300TB-FL-MF143C IN300TB-FL-MF143 IN300TB-FL-MF603 IN300TC-FL-MF143C IN300TC-FL-MF143 IN300TC-FL-MF603

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Regular price $8,313.99 $6,928.99
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Key Features

  • 40X to 400X magnification range using a quadruple lens turret for four unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 400X magnification range using a quadruple lens turret for four unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative monochrome CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 400X magnification range using a quadruple lens turret for four unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 6MP highly-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 600X expanded magnification range using a quadruple lens turret and additional 15X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 600X expanded magnification range using a quadruple lens turret and additional 15X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative monochrome CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 600X expanded magnification range using a quadruple lens turret and additional 15X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 6MP highly-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 800X expanded magnification range using a quadruple lens turret and additional 20X eyepieces for six unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 800X expanded magnification range using a quadruple lens turret and additional 20X eyepieces for six unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative monochrome CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 800X expanded magnification range using a quadruple lens turret and additional 20X eyepieces for six unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 6MP highly-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1000X expanded magnification range using a quadruple lens turret and additional 25X eyepieces for seven unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1000X expanded magnification range using a quadruple lens turret and additional 25X eyepieces for seven unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative monochrome CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1000X expanded magnification range using a quadruple lens turret and additional 25X eyepieces for seven unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 6MP highly-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux

The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

Transmitted Illumination

The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Phase Contrast

The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

Epi-fluorescence Illumination

For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

Fluorescence

Filters
Filter Set Excitation Filter Emission Filter
B 450-490nm 520nm
G 510-560nm 590nm
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Digital Imaging

The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Optical System Infinity-corrected
Head Trinocular, 45° incline
Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
Ocular Diameter 30mm
Ocular Diopters right ocular
Eyepieces 10X
Photo-port height-adjustable 23mm tube
Objective Lenses Infinity-corrected
Objective Turret Quadruple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Stage fixed-height, mechanical X-Y translation
Stage Dimensions 172mm x 242mm
Stage X-Y Travel Range 75mm x 40mm
Specimen Holders Terasaki plate, Petri dish
Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
Transmitted Illumination 12V 30W halogen
variable intensity
Condenser NA 0.3, iris diaphragm, filter slot
Power 110VAC 60Hz

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X plan fluor 0.15 28mm 1.5mm
10X plan fluor, phase-contrast 0.30 3mm 1.5mm
20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
40X plan fluor 0.60 2.23mm 1.5mm

Camera Specifications
Sensor Type Interline CCD (color)
Sensor Optical Format 2/3"
Active Pixels 1.4M (1360 x 1024)
Pixel Size 6.45µm x 6.45µm
Active Sensor Area 8.77mm x 6.6mm
Shutter global shutter
Sensitivity 1240mV @ 1/30s
Spectral Response 380-650nm with IR-cut filter
Capture Resolution and Maximum Framerate 15fps @ 1360x1024
Connectivity USB 3.0
Lens-mount C-mount
Compensating Lens 0.67X/0.75X

Software

OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One software CD
  • One heavy-duty case
  • The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (monochrome)
    Sensor Optical Format 2/3"
    Active Pixels 1.4M (1360 x 1024)
    Pixel Size 6.45µm x 6.45µm
    Active Sensor Area 8.77mm x 6.6mm
    Shutter global shutter
    Sensitivity 2000mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 25fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One software CD
  • One heavy-duty case
  • The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 1"
    Active Pixels 6M (1360 x 1024)
    Pixel Size 4.54µm x 4.54µm
    Active Sensor Area 12.48mm x 9.99mm
    Shutter global shutter
    Sensitivity 880mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 7.5fps @ 2748x2200
    14fps @ 2748x1092
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One software CD
  • One heavy-duty case
  • The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 15X eyepieces expand the maximum magnification to 600X with 8 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X, 15X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 2/3"
    Active Pixels 1.4M (1360 x 1024)
    Pixel Size 6.45µm x 6.45µm
    Active Sensor Area 8.77mm x 6.6mm
    Shutter global shutter
    Sensitivity 1240mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 15fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One software CD
  • One heavy-duty case
  • The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 15X eyepieces expand the maximum magnification to 600X with 8 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X, 15X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (monochrome)
    Sensor Optical Format 2/3"
    Active Pixels 1.4M (1360 x 1024)
    Pixel Size 6.45µm x 6.45µm
    Active Sensor Area 8.77mm x 6.6mm
    Shutter global shutter
    Sensitivity 2000mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 25fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One software CD
  • One heavy-duty case
  • The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 15X eyepieces expand the maximum magnification to 600X with 8 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X, 15X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 1"
    Active Pixels 6M (1360 x 1024)
    Pixel Size 4.54µm x 4.54µm
    Active Sensor Area 12.48mm x 9.99mm
    Shutter global shutter
    Sensitivity 880mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 7.5fps @ 2748x2200
    14fps @ 2748x1092
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One software CD
  • One heavy-duty case
  • The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 20X eyepieces expand the maximum magnification to 800X with 6 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X, 20X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 2/3"
    Active Pixels 1.4M (1360 x 1024)
    Pixel Size 6.45µm x 6.45µm
    Active Sensor Area 8.77mm x 6.6mm
    Shutter global shutter
    Sensitivity 1240mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 15fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One software CD
  • One heavy-duty case
  • The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 20X eyepieces expand the maximum magnification to 800X with 6 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X, 20X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (monochrome)
    Sensor Optical Format 2/3"
    Active Pixels 1.4M (1360 x 1024)
    Pixel Size 6.45µm x 6.45µm
    Active Sensor Area 8.77mm x 6.6mm
    Shutter global shutter
    Sensitivity 2000mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 25fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One software CD
  • One heavy-duty case
  • The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 20X eyepieces expand the maximum magnification to 800X with 6 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X, 20X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 1"
    Active Pixels 6M (1360 x 1024)
    Pixel Size 4.54µm x 4.54µm
    Active Sensor Area 12.48mm x 9.99mm
    Shutter global shutter
    Sensitivity 880mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 7.5fps @ 2748x2200
    14fps @ 2748x1092
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One software CD
  • One heavy-duty case
  • The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 25X eyepieces expand the maximum magnification to 1000X with 7 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X, 25X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 2/3"
    Active Pixels 1.4M (1360 x 1024)
    Pixel Size 6.45µm x 6.45µm
    Active Sensor Area 8.77mm x 6.6mm
    Shutter global shutter
    Sensitivity 1240mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 15fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One software CD
  • One heavy-duty case
  • The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 25X eyepieces expand the maximum magnification to 1000X with 7 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

    Transmitted Illumination

    The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Phase Contrast

    The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

    Epi-fluorescence Illumination

    For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

    Fluorescence

    Filters
    Filter Set Excitation Filter Emission Filter
    B 450-490nm 520nm
    G 510-560nm 590nm
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
    Ocular Diameter 30mm
    Ocular Diopters right ocular
    Eyepieces 10X, 25X
    Photo-port height-adjustable 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret Quadruple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 172mm x 242mm
    Stage X-Y Travel Range 75mm x 40mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 12V 30W halogen
    variable intensity
    Condenser NA 0.3, iris diaphragm, filter slot
    Power 110VAC 60Hz

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan fluor 0.15 28mm 1.5mm
    10X plan fluor, phase-contrast 0.30 3mm 1.5mm
    20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm
    40X plan fluor 0.60 2.23mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (monochrome)
    Sensor Optical Format 2/3"
    Active Pixels 1.4M (1360 x 1024)
    Pixel Size 6.45µm x 6.45µm
    Active Sensor Area 8.77mm x 6.6mm
    Shutter global shutter
    Sensitivity 2000mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 25fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview