AmScope IN480 Series Inverted 30W Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-600X Magnification with Quintuple Head and 2.8MP Color CCD USB 3.0 Camera AmScope IN480 Series Inverted 30W Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-600X Magnification with Quintuple Head and 12MP Color CCD USB 3.0 Camera AmScope IN480 Series Inverted 30W Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-900X Magnification with Quintuple Head and 2.8MP Color CCD USB 3.0 Camera AmScope IN480 Series Inverted 30W Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-900X Magnification with Quintuple Head and 12MP Color CCD USB 3.0 Camera AmScope IN480 Series Inverted 30W Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1200X Magnification with Quintuple Head and 2.8MP Color CCD USB 3.0 Camera AmScope IN480 Series Inverted 30W Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1200X Magnification with Quintuple Head and 12MP Color CCD USB 3.0 Camera AmScope IN480 Series Inverted 30W Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1500X Magnification with Quintuple Head and 2.8MP Color CCD USB 3.0 Camera AmScope IN480 Series Inverted 30W Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1500X Magnification with Quintuple Head and 12MP Color CCD USB 3.0 Camera

SKU: IN480T-FL-MF283 IN480T-FL-MF1203 IN480TA-FL-MF283 IN480TA-FL-MF1203 IN480TB-FL-MF283 IN480TB-FL-MF1203 IN480TC-FL-MF283 IN480TC-FL-MF1203

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$10,633.99
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Key Features

  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 2.8MP highly-sensative color CCD camera for low-light applications
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 12MP highly-sensative color CCD camera for low-light applications
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 2.8MP highly-sensative color CCD camera for low-light applications
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 12MP highly-sensative color CCD camera for low-light applications
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 2.8MP highly-sensative color CCD camera for low-light applications
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 12MP highly-sensative color CCD camera for low-light applications
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 2.8MP highly-sensative color CCD camera for low-light applications
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 12MP highly-sensative color CCD camera for low-light applications
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Digital Imaging

The highly-sensitive 2.8MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


Optical System Infinity-corrected
Reference Focal Length 220mm
Head Trinocular, 45° incline
Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter 30mm
Ocular Diopters left ocular
Eyepieces 10X
Photo-port CX-type height-adjustable, 23mm tube
Objective Lenses Infinity-corrected
Objective Turret quintuple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
Stage fixed-height, mechanical X-Y translation
Stage Dimensions 160mm x 250mm
stage extension: 70mm x 180mm
Stage X-Y Travel Range 120mm x 80mm
Specimen Holders Terasaki plate, Petri dish
Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
Transmitted Illumination 6V 30W halogen
variable intensity
centerable lamp
Condenser NA 0.3, centerable
Condenser Working Distance 72mm maximum
Phase Contrast Annuli 4X/10X and 20X/40X
Power 90-240VAC 50/60Hz wide-band

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X plan achromatic 0.13 10.4mm 1.5mm
10X plan achromatic 0.25 7.30mm 1.5mm
20X plan achromatic 0.40 6.79mm 1.5mm
40X plan achromatic 0.60 3.08mm 1.5mm
60X plan achromatic 0.70 1.71mm 1.5mm
10X phase-ring 0.25 7.30mm 1.5mm
20X phase-ring 0.40 6.80mm 1.5mm
40X phase-ring 0.60 3.08mm 1.5mm

Camera Specifications
Sensor Type Interline CCD (color)
Sensor Optical Format 2/3"
Active Pixels 2.8M (1938 x 1460)
Pixel Size 4.54µm x 4.54µm
Active Sensor Area 8.8mm x 6.63mm
Shutter global shutter
Sensitivity 800mV @ 1/30s
Spectral Response 380-650nm with IR-cut filter
Capture Resolution and Maximum Framerate 15fps @ 1360x1024
Connectivity USB 3.0
Lens-mount C-mount
Compensating Lens 0.67X/0.75X

Software

OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One 23mm C-mount reduction lens
  • Two port adapters: 30mm, 30.5mm
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 12MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 1"
    Active Pixels 12M (4284x2836)
    Pixel Size 3.1µm x 3.1µm
    Active Sensor Area 13.15mm x 8.77mm
    Shutter global shutter
    Sensitivity 420mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 3.6fps @ 4284x2836
    3.6fps @ 2124x1418
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 2.8MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 2/3"
    Active Pixels 2.8M (1938 x 1460)
    Pixel Size 4.54µm x 4.54µm
    Active Sensor Area 8.8mm x 6.63mm
    Shutter global shutter
    Sensitivity 800mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 15fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One 23mm C-mount reduction lens
  • Two port adapters: 30mm, 30.5mm
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 12MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 1"
    Active Pixels 12M (4284x2836)
    Pixel Size 3.1µm x 3.1µm
    Active Sensor Area 13.15mm x 8.77mm
    Shutter global shutter
    Sensitivity 420mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 3.6fps @ 4284x2836
    3.6fps @ 2124x1418
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 2.8MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 20X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 2/3"
    Active Pixels 2.8M (1938 x 1460)
    Pixel Size 4.54µm x 4.54µm
    Active Sensor Area 8.8mm x 6.63mm
    Shutter global shutter
    Sensitivity 800mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 15fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One 23mm C-mount reduction lens
  • Two port adapters: 30mm, 30.5mm
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 12MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 20X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 1"
    Active Pixels 12M (4284x2836)
    Pixel Size 3.1µm x 3.1µm
    Active Sensor Area 13.15mm x 8.77mm
    Shutter global shutter
    Sensitivity 420mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 3.6fps @ 4284x2836
    3.6fps @ 2124x1418
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 2.8MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 25X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 2/3"
    Active Pixels 2.8M (1938 x 1460)
    Pixel Size 4.54µm x 4.54µm
    Active Sensor Area 8.8mm x 6.63mm
    Shutter global shutter
    Sensitivity 800mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 15fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One 23mm C-mount reduction lens
  • Two port adapters: 30mm, 30.5mm
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 12MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 25X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 1"
    Active Pixels 12M (4284x2836)
    Pixel Size 3.1µm x 3.1µm
    Active Sensor Area 13.15mm x 8.77mm
    Shutter global shutter
    Sensitivity 420mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 3.6fps @ 4284x2836
    3.6fps @ 2124x1418
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One USB 3.0 cable
  • One software CD
  • Applications Clinic, Laboratory, Veterinary
    Camera Compatibility PC & Mac
    Camera Sensor Type CCD
    Camera Type USB 3.0
    Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
    Condenser Type Brightfield, Phase Contrast
    Education Level College, Teaching/Training
    Eyepiece Mounting Size 30mm
    Field of View Class Extreme Widefield
    Head Style Trinocular
    Illumination Bulb Count 1
    Illumination Power 30W, 100W
    Illumination Style Incident & Transmitted
    Illumination Type Halogen, Fluorescent
    Iris With Iris
    Magnification 40X-600X
    Magnification Type Multi-Power
    Objective Lens Power 4X, 10X, 20X, 40X, 60X
    Objective Mounting Size 20mm
    Optics Plan, Achromatic, Infinity
    Power Compatibility Wide Band
    product condition NEW
    Product Type Category Compound
    Applications Clinic, Laboratory, Veterinary
    Camera Compatibility PC & Mac
    Camera Sensor Type CCD
    Camera Type USB 3.0
    Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
    Condenser Type Brightfield, Phase Contrast
    Education Level College, Teaching/Training
    Eyepiece Mounting Size 30mm
    Field of View Class Extreme Widefield
    Head Style Trinocular
    Illumination Bulb Count 1
    Illumination Power 30W, 100W
    Illumination Style Incident & Transmitted
    Illumination Type Halogen, Fluorescent
    Iris With Iris
    Magnification 40X-600X
    Magnification Type Multi-Power
    Objective Lens Power 4X, 10X, 20X, 40X, 60X
    Objective Mounting Size 20mm
    Optics Plan, Achromatic, Infinity
    Power Compatibility Wide Band
    product condition NEW
    Product Type Category Compound
    Applications Clinic, Laboratory, Veterinary
    Camera Compatibility PC & Mac
    Camera Sensor Type CCD
    Camera Type USB 3.0
    Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
    Condenser Type Brightfield, Phase Contrast
    Education Level College, Teaching/Training
    Eyepiece Mounting Size 30mm
    Field of View Class Extreme Widefield
    Head Style Trinocular
    Illumination Bulb Count 1
    Illumination Power 30W, 100W
    Illumination Style Incident & Transmitted
    Illumination Type Halogen, Fluorescent
    Iris With Iris
    Magnification 40X-900X
    Magnification Type Multi-Power
    Objective Lens Power 4X, 10X, 20X, 40X, 60X
    Objective Mounting Size 20mm
    Optics Plan, Achromatic, Infinity
    Power Compatibility Wide Band
    product condition NEW
    Product Type Category Compound
    Applications Clinic, Laboratory, Veterinary
    Camera Compatibility PC & Mac
    Camera Sensor Type CCD
    Camera Type USB 3.0
    Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
    Condenser Type Brightfield, Phase Contrast
    Education Level College, Teaching/Training
    Eyepiece Mounting Size 30mm
    Field of View Class Extreme Widefield
    Head Style Trinocular
    Illumination Bulb Count 1
    Illumination Power 30W, 100W
    Illumination Style Incident & Transmitted
    Illumination Type Halogen, Fluorescent
    Iris With Iris
    Magnification 40X-900X
    Magnification Type Multi-Power
    Objective Lens Power 4X, 10X, 20X, 40X, 60X
    Objective Mounting Size 20mm
    Optics Plan, Achromatic, Infinity
    Power Compatibility Wide Band
    product condition NEW
    Product Type Category Compound
    Applications Clinic, Laboratory, Veterinary
    Camera Compatibility PC & Mac
    Camera Sensor Type CCD
    Camera Type USB 3.0
    Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
    Condenser Type Brightfield, Phase Contrast
    Education Level College, Teaching/Training
    Eyepiece Mounting Size 30mm
    Field of View Class Extreme Widefield
    Head Style Trinocular
    Illumination Bulb Count 1
    Illumination Power 30W, 100W
    Illumination Style Incident & Transmitted
    Illumination Type Halogen, Fluorescent
    Iris With Iris
    Magnification 40X-1200X
    Magnification Type Multi-Power
    Objective Lens Power 4X, 10X, 20X, 40X, 60X
    Objective Mounting Size 20mm
    Optics Plan, Achromatic, Infinity
    Power Compatibility Wide Band
    product condition NEW
    Product Type Category Compound
    Applications Clinic, Laboratory, Veterinary
    Camera Compatibility PC & Mac
    Camera Sensor Type CCD
    Camera Type USB 3.0
    Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
    Condenser Type Brightfield, Phase Contrast
    Education Level College, Teaching/Training
    Eyepiece Mounting Size 30mm
    Field of View Class Extreme Widefield
    Head Style Trinocular
    Illumination Bulb Count 1
    Illumination Power 30W, 100W
    Illumination Style Incident & Transmitted
    Illumination Type Halogen, Fluorescent
    Iris With Iris
    Magnification 40X-1200X
    Magnification Type Multi-Power
    Objective Lens Power 4X, 10X, 20X, 40X, 60X
    Objective Mounting Size 20mm
    Optics Plan, Achromatic, Infinity
    Power Compatibility Wide Band
    product condition NEW
    Product Type Category Compound
    Applications Clinic, Laboratory, Veterinary
    Camera Compatibility PC & Mac
    Camera Sensor Type CCD
    Camera Type USB 3.0
    Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
    Condenser Type Brightfield, Phase Contrast
    Education Level College, Teaching/Training
    Eyepiece Mounting Size 30mm
    Field of View Class Extreme Widefield
    Head Style Trinocular
    Illumination Bulb Count 1
    Illumination Power 30W, 100W
    Illumination Style Incident & Transmitted
    Illumination Type Halogen, Fluorescent
    Iris With Iris
    Magnification 40X-1500X
    Magnification Type Multi-Power
    Objective Lens Power 4X, 10X, 20X, 40X, 60X
    Objective Mounting Size 20mm
    Optics Plan, Achromatic, Infinity
    Power Compatibility Wide Band
    product condition NEW
    Product Type Category Compound
    Applications Clinic, Laboratory, Veterinary
    Camera Compatibility PC & Mac
    Camera Sensor Type CCD
    Camera Type USB 3.0
    Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
    Condenser Type Brightfield, Phase Contrast
    Education Level College, Teaching/Training
    Eyepiece Mounting Size 30mm
    Field of View Class Extreme Widefield
    Head Style Trinocular
    Illumination Bulb Count 1
    Illumination Power 30W, 100W
    Illumination Style Incident & Transmitted
    Illumination Type Halogen, Fluorescent
    Iris With Iris
    Magnification 40X-1500X
    Magnification Type Multi-Power
    Objective Lens Power 4X, 10X, 20X, 40X, 60X
    Objective Mounting Size 20mm
    Optics Plan, Achromatic, Infinity
    Power Compatibility Wide Band
    product condition NEW
    Product Type Category Compound

    TPG

    Enhance your microscopy experience by downloading the essential software for your microscope cameras. This software provides advanced features such as image capture, measurement tools, and editing capabilities, allowing you to maximize the potential of your microscope. Compatible with various camera models, it ensures seamless integration and optimal performance. Please use the software attached below to download the software and unlock the full capabilities of your microscope camera today.View our software licensing terms.

    Operating System Software
    Windows v.10.11.2024
    MAC AmScopeAmLite_20240408.dmg
    SDK TPG SDK (Optional) v.03.11.2024
    Linux AmScopeAmLite_x64_x84_20231115.tar

    AmScope products are warranted against manufacturer’s defects in material and workmanship from the original date of purchase, for the following years: 


    • Five Year Warranty for Microscopes: All our microscopes come with a five (5) year factory warranty against manufacturing defects, covering labor and parts. 
    • Ten Year Warranty for Euromex Microscopes: All our Euromex microscopes come with a ten (10) year factory warranty against manufacturing defects, covering labor and parts.
    • One Year Warranty for Telescopes: All our telescopes come with a one (1) year warranty, except when specified in the description. 
    • One Year Warranty for Accessories: All our microscope accessories (USB cameras, illuminators, illumination, dust covers, and etc.) come with a one (1) year warranty, except when specified in the description.
    • One Year Warranty for Items labeled as “Product Ships From Manufacturer”: All products that are shipped directly from the manufacturer come with a one (1) year warranty, except when specified in the description. 

    Warranty does not apply for consumable accessories and parts such as bulbs, batteries, fuses, cords, optical components or any add-on accessories such as mechanical specimen holders that are not built into the microscope stage as an integral part of the original manufacture, etc. Warranty does not cover microscopes, lenses cameras or other accessories that have become inoperable due to dirt or damage due to misuse or lack of maintenance. 

    Note: Buyers are responsible for return shipping and handling cost for warranty services. Our warranty covers parts and labor only.

    The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 2.8MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 2/3"
    Active Pixels 2.8M (1938 x 1460)
    Pixel Size 4.54µm x 4.54µm
    Active Sensor Area 8.8mm x 6.63mm
    Shutter global shutter
    Sensitivity 800mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 15fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One 23mm C-mount reduction lens
  • Two port adapters: 30mm, 30.5mm
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 12MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 1"
    Active Pixels 12M (4284x2836)
    Pixel Size 3.1µm x 3.1µm
    Active Sensor Area 13.15mm x 8.77mm
    Shutter global shutter
    Sensitivity 420mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 3.6fps @ 4284x2836
    3.6fps @ 2124x1418
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 2.8MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 2/3"
    Active Pixels 2.8M (1938 x 1460)
    Pixel Size 4.54µm x 4.54µm
    Active Sensor Area 8.8mm x 6.63mm
    Shutter global shutter
    Sensitivity 800mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 15fps @ 1360x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.67X/0.75X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One 23mm C-mount reduction lens
  • Two port adapters: 30mm, 30.5mm
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 12MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Interline CCD (color)
    Sensor Optical Format 1"
    Active Pixels 12M (4284x2836)
    Pixel Size 3.1µm x 3.1µm
    Active Sensor Area 13.15mm x 8.77mm
    Shutter global shutter
    Sensitivity 420mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 3.6fps @ 4284x2836
    3.6fps @ 2124x1418
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One USB 3.0 CCD C-mount camera
  • One USB 3.0 cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 2.8MP color CCD digital camera provides a low-light imaging solution, ideal for fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 20X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm