40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 1.5MP Global Shutter Camera 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 2.3MP Global Shutter Camera 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 3.1MP Global Shutter Camera 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 5.0MP Global Shutter Camera 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 3.1MP Global Shutter Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination9.0MP Global Shutter Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 1.5MP Global Shutter Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 2.3MP Global Shutter Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 3.1MP Global Shutter Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 5.0MP Global Shutter Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 3.1MP Global Shutter Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination9.0MP Global Shutter Camera 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 1.5MP Global Shutter Camera 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 2.3MP Global Shutter Camera 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 3.1MP Global Shutter Camera 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 5.0MP Global Shutter Camera 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 3.1MP Global Shutter Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination9.0MP Global Shutter Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 1.5MP Global Shutter Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 2.3MP Global Shutter Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 3.1MP Global Shutter Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 5.0MP Global Shutter Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination and 3.1MP Global Shutter Camera

SKU: IN480T-FL IN480T-FL-MG3 IN480T-FL-2MG3 IN480T-FL-3MG3 IN480T-FL-5MG3 IN480T-FL-9MG3 IN480T-FL-3MGM3 IN480TA-FL IN480TA-FL-MG3 IN480TA-FL-2MG3 IN480TA-FL-3MG3 IN480TA-FL-5MG3 IN480TA-FL-9MG3 IN480TA-FL-3MGM3 IN480TB-FL IN480TB-FL-MG3 IN480TB-FL-2MG3 IN480TB-FL-3MG3 IN480TB-FL-5MG3 IN480TB-FL-9MG3 IN480TB-FL-3MGM3 IN480TC-FL IN480TC-FL-MG3 IN480TC-FL-2MG3 IN480TC-FL-3MG3 IN480TC-FL-5MG3 IN480TC-FL-9MG3 IN480TC-FL-3MGM3

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Regular price $13,813.99 $9,945.99
>> How to Buy A Microscope

SELECTING A COMPOUND MICROSCOPE is as easy as 1-2-3...

Select the type of microscope you need or your industry

Select by type of microscope - Our Compound Microscope Assortment has been divided into major categories to make your shopping easy.

These categories include:

Brightfield, Darkfield, Phase-Contrast, Inverted and others

Choose a business application or industry to find the right compound microscope. Major industries or applicatins include:

Veterinary, Laboratory Use, Teaching, Metallurgical, Gemology and many others

Select a Magnification

AmScope offers an industry-leading selection of magnifications. All products are in-stock and usually ship the same day ordered

Select a Camera

We offer a wide variety of Cameras for every application - from student to professional use:

Select a type of camera - Eyepiece, USB 2.0 or USB 3.0 - to name the most popular choices

Choose the Megapixels of the camera - the higher the megapixel count on a camera sensor the greater the amount of detail that can be captured in an image

Magnification Power*
Camera Type*
Camera Pixel*

The Higher the Magnification - The More Detail You'll See

Magnification 40x
Magnification 1000x
Magnification 2500x

Magnification is the ability of your microscope to make small objects seem larger. As the magnification increases the area being viewed will be decrease is size but the detail of the area being viewed will improve.

Amscope microscopes come with multiple magnifications to aid you in finding the proper magnification for viewing each specimen.

Camera Connection Types

When comparing 2.0 and 3.0 there are few major differences. First the transfer rates. USB 2.0 offers transfer rates of 480 Mbps and USB 3.0 offers transfer rates of 4.8 Gbps - that's 10 times faster.

Camera Resolution

The most important factor in ensuring successful microscopic imaging is choosing the appropriate optics and camera for your application that gives you more data and higher image quality. When selecting a microscope camera, it is best to base your decision on the most important requirements for your microscopic observation.

* Required Fields

Key Features

  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes an ultra-sensitive 0.4MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 2.3MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 3.1MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 5MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 9MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive monochrome 3.1MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes an ultra-sensitive 0.4MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 2.3MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 3.1MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 5MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 9MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive monochrome 3.1MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes an ultra-sensitive 0.4MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 2.3MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 3.1MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 5MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 9MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive monochrome 3.1MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes an ultra-sensitive 0.4MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 2.3MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 3.1MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 5MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 9MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive monochrome 3.1MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Specifications

Technical drawings with measurements (in mm)


Optical System Infinity-corrected
Reference Focal Length 220mm
Head Trinocular, 45° incline
Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter 30mm
Ocular Diopters left ocular
Eyepieces 10X
Photo-port CX-type height-adjustable, 23mm tube
Objective Lenses Infinity-corrected
Objective Turret quintuple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
Stage fixed-height, mechanical X-Y translation
Stage Dimensions 160mm x 250mm
stage extension: 70mm x 180mm
Stage X-Y Travel Range 120mm x 80mm
Specimen Holders Terasaki plate, Petri dish
Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
Transmitted Illumination 6V 30W halogen
variable intensity
centerable lamp
Condenser NA 0.3, centerable
Condenser Working Distance 72mm maximum
Phase Contrast Annuli 4X/10X and 20X/40X
Power 90-240VAC 50/60Hz wide-band

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X plan achromatic 0.13 10.4mm 1.5mm
10X plan achromatic 0.25 7.30mm 1.5mm
20X plan achromatic 0.40 6.79mm 1.5mm
40X plan achromatic 0.60 3.08mm 1.5mm
60X plan achromatic 0.70 1.71mm 1.5mm
10X phase-ring 0.25 7.30mm 1.5mm
20X phase-ring 0.40 6.80mm 1.5mm
40X phase-ring 0.60 3.08mm 1.5mm

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 0.4MP monochrome camera is the most sensitive camera we carry, with extremely fast maximum framerates. It uses a cutting-edge Sony global-shutter sensor which is designed to excel in low-light, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (monochrome)
    Sensor Optical Format 1/3"
    Active Pixels 0.4M (720 x 540)
    Pixel Size 6.9µm x 6.9µm
    Active Sensor Area 4.97mm x 3.73mm
    Shutter global shutter
    Sensitivity 7469mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 520fps @ 720x540*

    *Maximum framerate is for preview only. Recorded framerate will depend on computer hardware, including CPU, GPU, and hard drive. Software codecs may limit recorded framerate depending on compression.

    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.37X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.37X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 2.3MP digital camera uses a cutting-edge global-shutter CMOS sensor which provides better motion-rendering, eliminating rolling-shutter artifacts. The new design based partly on CCD architecture provides extremely-high sensitivity, ideal for fluorescence microscopy and other low-light applications. In brightfield applications, the camera can record up to 30fps. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (color)
    Sensor Optical Format 1/1.2"
    Active Pixels 2.3M (1920 x 1200)
    Pixel Size 5.86µm x 5.86µm
    Active Sensor Area 11.25mm x 7.03mm
    Shutter global shutter
    Sensitivity 1016mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 30fps @ 1920x1200
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.37X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 3.1MP camera uses a cutting-edge Sony global-shutter sensor. The camera is designed to excel in low-light with high maximum framerates, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (color)
    Sensor Optical Format 1/1.8"
    Active Pixels 3.1M (2048 x 1536)
    Pixel Size 3.45µm x 3.5µm
    Active Sensor Area 7.07mm x 5.3mm
    Shutter global shutter
    Sensitivity 1146mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 53fps @ 2048x1536
    85fps @ 1024x768
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.5X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 5MP camera uses a cutting-edge Sony global-shutter sensor. The camera is designed to excel in low-light with high maximum framerates, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (color)
    Sensor Optical Format 2/3"
    Active Pixels 5M (2448 x 2048)
    Pixel Size 3.45µm x 3.5µm
    Active Sensor Area 8.45mm x 7.07mm
    Shutter global shutter
    Sensitivity 1146mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 35fps @ 2448x2048
    88fps @ 1224x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.5X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 9MP camera uses a cutting-edge 1"-format Sony global-shutter sensor. The camera is designed to excel in low-light with high maximum framerates, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (color)
    Sensor Optical Format 1"
    Active Pixels 9M (4096 x 2160)
    Pixel Size 3.45µm x 3.45µm
    Active Sensor Area 14.13mm x 7.45mm
    Shutter global shutter
    Sensitivity 1146mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 34fps @ 4096x2160
    60fps @ 2048x1080
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 3.1MP camera uses a cutting-edge monochrome Sony global-shutter sensor. The monochrome sensor is more efficient due to the lack of a color-filter array, maximizing the amount of light absorbed. The camera is designed to excel in low-light with high maximum framerates, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (monochrome)
    Sensor Optical Format 1/1.8"
    Active Pixels 3.1M (2048 x 1536)
    Pixel Size 3.45µm x 3.5µm
    Active Sensor Area 7.07mm x 5.3mm
    Shutter global shutter
    Sensitivity 1146mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 53fps @ 2048x1536
    85fps @ 1024x768
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.5X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 0.4MP monochrome camera is the most sensitive camera we carry, with extremely fast maximum framerates. It uses a cutting-edge Sony global-shutter sensor which is designed to excel in low-light, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (monochrome)
    Sensor Optical Format 1/3"
    Active Pixels 0.4M (720 x 540)
    Pixel Size 6.9µm x 6.9µm
    Active Sensor Area 4.97mm x 3.73mm
    Shutter global shutter
    Sensitivity 7469mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 520fps @ 720x540*

    *Maximum framerate is for preview only. Recorded framerate will depend on computer hardware, including CPU, GPU, and hard drive. Software codecs may limit recorded framerate depending on compression.

    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.37X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.37X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 2.3MP digital camera uses a cutting-edge global-shutter CMOS sensor which provides better motion-rendering, eliminating rolling-shutter artifacts. The new design based partly on CCD architecture provides extremely-high sensitivity, ideal for fluorescence microscopy and other low-light applications. In brightfield applications, the camera can record up to 30fps. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (color)
    Sensor Optical Format 1/1.2"
    Active Pixels 2.3M (1920 x 1200)
    Pixel Size 5.86µm x 5.86µm
    Active Sensor Area 11.25mm x 7.03mm
    Shutter global shutter
    Sensitivity 1016mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 30fps @ 1920x1200
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.37X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 3.1MP camera uses a cutting-edge Sony global-shutter sensor. The camera is designed to excel in low-light with high maximum framerates, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (color)
    Sensor Optical Format 1/1.8"
    Active Pixels 3.1M (2048 x 1536)
    Pixel Size 3.45µm x 3.5µm
    Active Sensor Area 7.07mm x 5.3mm
    Shutter global shutter
    Sensitivity 1146mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 53fps @ 2048x1536
    85fps @ 1024x768
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.5X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 5MP camera uses a cutting-edge Sony global-shutter sensor. The camera is designed to excel in low-light with high maximum framerates, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (color)
    Sensor Optical Format 2/3"
    Active Pixels 5M (2448 x 2048)
    Pixel Size 3.45µm x 3.5µm
    Active Sensor Area 8.45mm x 7.07mm
    Shutter global shutter
    Sensitivity 1146mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 35fps @ 2448x2048
    88fps @ 1224x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.5X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The highly-sensitive 9MP camera uses a cutting-edge 1"-format Sony global-shutter sensor. The camera is designed to excel in low-light with high maximum framerates, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (color)
    Sensor Optical Format 1"
    Active Pixels 9M (4096 x 2160)
    Pixel Size 3.45µm x 3.45µm
    Active Sensor Area 14.13mm x 7.45mm
    Shutter global shutter
    Sensitivity 1146mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 34fps @ 4096x2160
    60fps @ 2048x1080
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 3.1MP camera uses a cutting-edge monochrome Sony global-shutter sensor. The monochrome sensor is more efficient due to the lack of a color-filter array, maximizing the amount of light absorbed. The camera is designed to excel in low-light with high maximum framerates, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type CMOS (monochrome)
    Sensor Optical Format 1/1.8"
    Active Pixels 3.1M (2048 x 1536)
    Pixel Size 3.45µm x 3.5µm
    Active Sensor Area 7.07mm x 5.3mm
    Shutter global shutter
    Sensitivity 1146mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 53fps @ 2048x1536
    85fps @ 1024x768
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.5X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 20X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    The ultra-sensitive 0.4MP monochrome camera is the most sensitive camera we carry, with extremely fast maximum framerates. It uses a cutting-edge Sony global-shutter sensor which is designed to excel in low-light, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 20X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm