AmScope IN480 Series Inverted Infinity-corrected Phase-contrast Trinocular Compound Microscope 40X-1500X Magnification with Koehler Illumination and 5MP CCD Camera

SKU: IN480TC-FL-5MF

Availability: In Stock

$10,274.99

Key Features

  • Designed for fluorescence microscopy, this microscope uses a high-power arc-lamp to cover a wide excitation range.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • Five plan-achromatic objective lenses and two sets of eyepieces provide nine unique magnifications ranging from 40X to 1500X
  • The infinity-corrected optical system eliminates certain optical errors when using filters and other in-line components, ensuring highly-precise images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • Includes a highly-sensitive 5MP color CCD camera and specialized microscopy software for PC

The IN480T-FL provides high-resolution optics and a versatile dual-illumination system with Koehler transmitted lighting for brightfield and phase-contrast, and wide-spectrum episcopic lighting for fluorescence. The stable and ergonomic inverted form accommodates larger specimen containers, with multiple specimen templates and mechanical stage controls to improve workflow.

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging.

The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly-precise images. Five brightfield objective lenses and two sets of eyepieces provide a wide magnification range from 40X to 1500X, with plan-achromatic corrections for high resolution, accurate colors, and edge-to-edge sharpness. Additionally, three phase-ring equipped objective lenses can be used for phase-contrast observation.

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

The highly-sensitive 5MP color CCD camera captures low-light still images and video, and allows you to view a live stream on your PC. The included Windows software offers image-development and measurement tools. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images.

This microscope operates on 90-240V wide-band voltage for use in multiple international regions.

Fluorescence

Filters
Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Specifications

Optical System Infinity-corrected
Head Trinocular, 45° incline
Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter 30mm
Eyepieces 10X FN22mm plan wide-field, 25X wide-field
Photo-port CX-type height-adjustable, 23mm tube
Objective Turret quintuple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
Stage fixed-height, mechanical X-Y translation
Stage Dimensions 160mm x 250mm
stage extension: 70mm x 180mm
Stage X-Y Travel Range 120mm x 80mm
Specimen Holders Terasaki plate, Petri dish
Transmitted Illumination 6V 30W halogen
variable intensity
centerable lamp
Condenser NA 0.3, centerable
Condenser Working Distance 72mm maximum
Phase Contrast Annuli 4X/10X and 20X/40X
Power 90-240VAC 50/60Hz wide-band

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X plan achromatic 0.13 10.4mm 1.5mm
10X plan achromatic 0.25 7.30mm 1.5mm
20X plan achromatic 0.40 6.79mm 1.5mm
40X plan achromatic 0.60 3.08mm 1.5mm
60X plan achromatic 0.70 1.71mm 1.5mm
10X phase-ring 0.25 7.30mm 1.5mm
20X phase-ring 0.40 6.80mm 1.5mm
40X phase-ring 0.60 3.08mm 1.5mm

Magnification Chart

Eyepiece Objective
4X 10X 20X 40X 60X
10X 40X 100X 200X 400X 600X
25X 100X 250X 500X 1000X 1500X

Camera Specifications

Sensor Type CCD (color)
Sensor Size 2/3" format (9.74mm x 7.96mm)
Pixels 5M
Pixel Size 3.4µm x 3.4µm
Transfer Method Interline Progressive Scan (Global Shutter)
Sensitivity 280mV @ 1/30s (f5.6)
Smear -92dB
Dark Noise 16mV @ 1/30s
Frame Rate(s) 3fps @ 2580x1944
10fps @ 1280x932
Connectivity USB 2.0
Lens-mount C-mount
Compensating Lens
Cooling Type Peltier Thermoelectric
Cooling -30°C below ambient

Software

OS Requirements Windows (32/64 bit) XP/Vista/7/8/10
Features
Windows Version
Live video preview
Captures still images and video
Exposure and white-balance controls
Image processing
Measurement tools, including lines, arcs, polygons

Contents

  • One microscope
  • One 23mm photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair of 10X eyepieces
  • One pair of 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One camera
  • One camera power adapter
  • One 0.65X C-mount photo port
  • One USB cable
  • compatibility PC & Mac
    illuminatio style Incident & Transmitted
    illumination style Incident & Transmitted
    Manufacturer AmScope
    microscope application Industrial Inspection, Clinic, Veterinary, Laboratory
    microscope camera pixel 5MP
    microscope category Special
    microscope color White
    microscope condenser design Kohler
    microscope condenser type Brightfield, Phase Contrast
    microscope eyepiece power 10X, 25X
    microscope eyepiece size 30mm
    microscope field view Extreme Widefield
    microscope head style Trinocular
    microscope light power 30W, 100W
    microscope light shape Built-in
    microscope light type Halogen, Fluorescent
    microscope magnifying power 40X to 1500X
    microscope number of bulbs 2
    microscope objective power 4X, 10X, 20X, 40X, 60X
    microscope objective size 20mm
    microscope optics Infinity, Achromatic, Plan
    microscope power supply 110V, 220V
    microscope sensor CCD
    microscope specialty Phase Contrast, EPI-Fluorescence, Inverted
    microscope stage div 1.00mm
    ms illuminatiostyle Incident & Transmitted
    optics Infinity, Achromatic, Plan
    product condition NEW
    prop 65 Prop 65 Warning
    vendor AmScope

    TPG

    Enhance your microscopy experience by downloading the essential software for your microscope cameras. This software provides advanced features such as image capture, measurement tools, and editing capabilities, allowing you to maximize the potential of your microscope. Compatible with various camera models, it ensures seamless integration and optimal performance. Please use the software attached below to download the software and unlock the full capabilities of your microscope camera today.View our software licensing terms.

    Operating System Software
    Windows v.10.11.2024
    MAC AmScopeAmLite_20240408.dmg
    SDK TPG SDK (Optional) v.03.11.2024
    Linux AmScopeAmLite_x64_x84_20231115.tar

    AmScope products are warranted against manufacturer’s defects in material and workmanship from the original date of purchase, for the following years: 


    • Five Year Warranty for Microscopes: All our microscopes come with a five (5) year factory warranty against manufacturing defects, covering labor and parts. 
    • Ten Year Warranty for Euromex Microscopes: All our Euromex microscopes come with a ten (10) year factory warranty against manufacturing defects, covering labor and parts.
    • One Year Warranty for Telescopes: All our telescopes come with a one (1) year warranty, except when specified in the description. 
    • One Year Warranty for Accessories: All our microscope accessories (USB cameras, illuminators, illumination, dust covers, and etc.) come with a one (1) year warranty, except when specified in the description.
    • One Year Warranty for Items labeled as “Product Ships From Manufacturer”: All products that are shipped directly from the manufacturer come with a one (1) year warranty, except when specified in the description. 

    Warranty does not apply for consumable accessories and parts such as bulbs, batteries, fuses, cords, optical components or any add-on accessories such as mechanical specimen holders that are not built into the microscope stage as an integral part of the original manufacture, etc. Warranty does not cover microscopes, lenses cameras or other accessories that have become inoperable due to dirt or damage due to misuse or lack of maintenance. 

    Note: Buyers are responsible for return shipping and handling cost for warranty services. Our warranty covers parts and labor only.

    The IN480T-FL provides high-resolution optics and a versatile dual-illumination system with Koehler transmitted lighting for brightfield and phase-contrast, and wide-spectrum episcopic lighting for fluorescence. The stable and ergonomic inverted form accommodates larger specimen containers, with multiple specimen templates and mechanical stage controls to improve workflow.

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging.

    The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly-precise images. Five brightfield objective lenses and two sets of eyepieces provide a wide magnification range from 40X to 1500X, with plan-achromatic corrections for high resolution, accurate colors, and edge-to-edge sharpness. Additionally, three phase-ring equipped objective lenses can be used for phase-contrast observation.

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    The highly-sensitive 5MP color CCD camera captures low-light still images and video, and allows you to view a live stream on your PC. The included Windows software offers image-development and measurement tools. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images.

    This microscope operates on 90-240V wide-band voltage for use in multiple international regions.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Eyepieces 10X FN22mm plan wide-field, 25X wide-field
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Magnification Chart

    Eyepiece Objective
    4X 10X 20X 40X 60X
    10X 40X 100X 200X 400X 600X
    25X 100X 250X 500X 1000X 1500X

    Camera Specifications

    Sensor Type CCD (color)
    Sensor Size 2/3" format (9.74mm x 7.96mm)
    Pixels 5M
    Pixel Size 3.4µm x 3.4µm
    Transfer Method Interline Progressive Scan (Global Shutter)
    Sensitivity 280mV @ 1/30s (f5.6)
    Smear -92dB
    Dark Noise 16mV @ 1/30s
    Frame Rate(s) 3fps @ 2580x1944
    10fps @ 1280x932
    Connectivity USB 2.0
    Lens-mount C-mount
    Compensating Lens
    Cooling Type Peltier Thermoelectric
    Cooling -30°C below ambient

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Exposure and white-balance controls
    Image processing
    Measurement tools, including lines, arcs, polygons

    Contents

  • One microscope
  • One 23mm photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair of 10X eyepieces
  • One pair of 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One camera
  • One camera power adapter
  • One 0.65X C-mount photo port
  • One USB cable