40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 1.2MP Back-illuminated CMOS USB 3.0 Camera Inverted Infinity-Corrected Fluorescence Trinocular Microscope w/Global Shutter Digital Camera 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 3.1MP Back-illuminated CMOS USB 3.0 Camera 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 6.3MP Back-illuminated CMOS USB 3.0 Camera 40X-600X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 12MP Back-illuminated CMOS USB 3.0 Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination9.0MP Global Shutter Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 1.2MP Back-illuminated CMOS USB 3.0 Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 2MP Back-illuminated CMOS USB 3.0 Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 3.1MP Back-illuminated CMOS USB 3.0 Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 6.3MP Back-illuminated CMOS USB 3.0 Camera 40X-900X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 12MP Back-illuminated CMOS USB 3.0 Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination9.0MP Global Shutter Camera 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 1.2MP Back-illuminated CMOS USB 3.0 Camera 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 2MP Back-illuminated CMOS USB 3.0 Camera 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 3.1MP Back-illuminated CMOS USB 3.0 Camera 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 6.3MP Back-illuminated CMOS USB 3.0 Camera 40X-1200X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 12MP Back-illuminated CMOS USB 3.0 Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Microscope w/Kohler Illumination9.0MP Global Shutter Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 1.2MP Back-illuminated CMOS USB 3.0 Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 2MP Back-illuminated CMOS USB 3.0 Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 3.1MP Back-illuminated CMOS USB 3.0 Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 6.3MP Back-illuminated CMOS USB 3.0 Camera 40X-1500X Trinocular Inverted Infinity-Corrected Phase-Contrast Fluorescence Microscope w/Kohler Illumination and 12MP Back-illuminated CMOS USB 3.0 Camera

SKU: IN480T-FL IN480T-FL-MBI3 IN480T-FL-2MBI3 IN480T-FL-3MBI3 IN480T-FL-6MBI3 IN480T-FL-12MBI3 IN480T-FL-20MBI3 IN480TA-FL IN480TA-FL-MBI3 IN480TA-FL-2MBI3 IN480TA-FL-3MBI3 IN480TA-FL-6MBI3 IN480TA-FL-12MBI3 IN480TA-FL-20MBI3 IN480TB-FL IN480TB-FL-MBI3 IN480TB-FL-2MBI3 IN480TB-FL-3MBI3 IN480TB-FL-6MBI3 IN480TB-FL-12MBI3 IN480TB-FL-20MBI3 IN480TC-FL IN480TC-FL-MBI3 IN480TC-FL-2MBI3 IN480TC-FL-3MBI3 IN480TC-FL-6MBI3 IN480TC-FL-12MBI3 IN480TC-FL-20MBI3

Availability: In Stock In Stock In Stock In Stock In Stock In Stock Out of Stock In Stock In Stock In Stock In Stock In Stock In Stock Out of Stock In Stock In Stock In Stock In Stock In Stock In Stock Out of Stock In Stock In Stock In Stock In Stock In Stock In Stock Out of Stock

Regular price $13,813.99 $9,945.99
>> How to Buy A Microscope

SELECTING A COMPOUND MICROSCOPE is as easy as 1-2-3...

Select the type of microscope you need or your industry

Select by type of microscope - Our Compound Microscope Assortment has been divided into major categories to make your shopping easy.

These categories include:

Brightfield, Darkfield, Phase-Contrast, Inverted and others

Choose a business application or industry to find the right compound microscope. Major industries or applicatins include:

Veterinary, Laboratory Use, Teaching, Metallurgical, Gemology and many others

Select a Magnification

AmScope offers an industry-leading selection of magnifications. All products are in-stock and usually ship the same day ordered

Select a Camera

We offer a wide variety of Cameras for every application - from student to professional use:

Select a type of camera - Eyepiece, USB 2.0 or USB 3.0 - to name the most popular choices

Choose the Megapixels of the camera - the higher the megapixel count on a camera sensor the greater the amount of detail that can be captured in an image

Magnification Power*
Camera Type*
Pixel*

The Higher the Magnification - The More Detail You'll See

Magnification 40x
Magnification 1000x
Magnification 2500x

Magnification is the ability of your microscope to make small objects seem larger. As the magnification increases the area being viewed will be decrease is size but the detail of the area being viewed will improve.

Amscope microscopes come with multiple magnifications to aid you in finding the proper magnification for viewing each specimen.

Camera Connection Types

When comparing 2.0 and 3.0 there are few major differences. First the transfer rates. USB 2.0 offers transfer rates of 480 Mbps and USB 3.0 offers transfer rates of 4.8 Gbps - that's 10 times faster.

Camera Resolution

The most important factor in ensuring successful microscopic imaging is choosing the appropriate optics and camera for your application that gives you more data and higher image quality. When selecting a microscope camera, it is best to base your decision on the most important requirements for your microscopic observation.

* Required Fields

Key Features

  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included ultra-sensitive 1.2MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included ultra-sensitive 2MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included highly-sensitive 6.3MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included sensitive 12MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included back-illuminated 20MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included ultra-sensitive 1.2MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included ultra-sensitive 2MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included highly-sensitive 3.1MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included highly-sensitive 6.3MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included sensitive 12MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included back-illuminated 20MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included ultra-sensitive 1.2MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included ultra-sensitive 2MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included highly-sensitive 3.1MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included highly-sensitive 6.3MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included sensitive 12MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included back-illuminated 20MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included ultra-sensitive 1.2MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included ultra-sensitive 2MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included highly-sensitive 3.1MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included highly-sensitive 6.3MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included sensitive 12MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included back-illuminated 20MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Specifications

Technical drawings with measurements (in mm)


Optical System Infinity-corrected
Reference Focal Length 220mm
Head Trinocular, 45° incline
Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter 30mm
Ocular Diopters left ocular
Eyepieces 10X
Photo-port CX-type height-adjustable, 23mm tube
Objective Lenses Infinity-corrected
Objective Turret quintuple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
Stage fixed-height, mechanical X-Y translation
Stage Dimensions 160mm x 250mm
stage extension: 70mm x 180mm
Stage X-Y Travel Range 120mm x 80mm
Specimen Holders Terasaki plate, Petri dish
Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
Transmitted Illumination 6V 30W halogen
variable intensity
centerable lamp
Condenser NA 0.3, centerable
Condenser Working Distance 72mm maximum
Phase Contrast Annuli 4X/10X and 20X/40X
Power 90-240VAC 50/60Hz wide-band

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X plan achromatic 0.13 10.4mm 1.5mm
10X plan achromatic 0.25 7.30mm 1.5mm
20X plan achromatic 0.40 6.79mm 1.5mm
40X plan achromatic 0.60 3.08mm 1.5mm
60X plan achromatic 0.70 1.71mm 1.5mm
10X phase-ring 0.25 7.30mm 1.5mm
20X phase-ring 0.40 6.80mm 1.5mm
40X phase-ring 0.60 3.08mm 1.5mm

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 1.2MP USB3.0 camera. With extraordinary sensitivity and high framerates, the MU123-FL provides high-definition imaging for low-light applications, including fluorescence and darkfield. Using a cutting-edge 1.2MP back-illuminated CMOS sensor with an incredible 2040mV sensitivity (1/30s), high gain-levels can be used for highly-responsive realtime video. A 0.37X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/3"
    Active Pixels 1.2M (1280 x 960)
    Pixel Size 3.75µm x 3.75µm
    Active Sensor Area 4.80mm x 3.60mm
    Shutter electronic rolling shutter
    Sensitivity 2040mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 60fps @ 1280x960
    120fps @ 640x480
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.37X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.37X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 2MP USB3.0 camera. With extraordinary sensitivity and a maximum framerate of 125fps, the MU203-BI provides high-definition imaging for low-light applications, including fluorescence and darkfield. Using a cutting-edge 2MP back-illuminated CMOS sensor with an incredible 2350mV sensitivity (1/30s), high gain-levels can be used for highly-responsive realtime video. A 0.37X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/2.8"
    Active Pixels 2M (1920 x 1080)
    Pixel Size 2.9µm x 2.9µm
    Active Sensor Area 5.57mm x 3.13mm
    Shutter electronic rolling shutter
    Sensitivity 1300mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 125fps @ 1920x1080
    Connectivity USB 3.0
    Lens-mount C-mount
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.5X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 6.3MP USB3.0 camera. The highly-sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. A 0.5X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/1.8"
    Active Pixels 6.3M (3072 x 2048)
    Pixel Size 2.4µm x 2.4µm
    Active Sensor Area 7.37mm x 4.92mm
    Shutter electronic rolling shutter
    Sensitivity 425mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 30 fps @ 3072x2048, 38fps @ 1536x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.5X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 12MP USB3.0 camera. The sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. A 0.75X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/1.7"
    Active Pixels 12M (4000 x 3000)
    Pixel Size 1.85µm x 1.85µm
    Active Sensor Area 7.4mm x 5.55mm
    Shutter electronic rolling shutter
    Sensitivity 280mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 25fps @ 4000x3000, 50fps @ 2048x1080
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.75X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.75X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included high-resolution 20MP USB3.0 camera. The sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1"
    Active Pixels 20M (5440 x 3648)
    Pixel Size 2.4µm x 2.4µm
    Active Sensor Area 13.06mm x 8.76mm
    Shutter electronic rolling shutter
    Sensitivity 462mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 15fps @ 5440x3648, 50fps @ 2736x1824, 60fps @ 1824x1216
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 1.2MP USB3.0 camera. With extraordinary sensitivity and high framerates, the MU123-FL provides high-definition imaging for low-light applications, including fluorescence and darkfield. Using a cutting-edge 1.2MP back-illuminated CMOS sensor with an incredible 2040mV sensitivity (1/30s), high gain-levels can be used for highly-responsive realtime video. A 0.37X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/3"
    Active Pixels 1.2M (1280 x 960)
    Pixel Size 3.75µm x 3.75µm
    Active Sensor Area 4.80mm x 3.60mm
    Shutter electronic rolling shutter
    Sensitivity 2040mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 60fps @ 1280x960
    120fps @ 640x480
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.37X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.37X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 2MP USB3.0 camera. With extraordinary sensitivity and a maximum framerate of 125fps, the MU203-BI provides high-definition imaging for low-light applications, including fluorescence and darkfield. Using a cutting-edge 2MP back-illuminated CMOS sensor with an incredible 2350mV sensitivity (1/30s), high gain-levels can be used for highly-responsive realtime video. A 0.37X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/2.8"
    Active Pixels 2M (1920 x 1080)
    Pixel Size 2.9µm x 2.9µm
    Active Sensor Area 5.57mm x 3.13mm
    Shutter electronic rolling shutter
    Sensitivity 1300mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 125fps @ 1920x1080
    Connectivity USB 3.0
    Lens-mount C-mount
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.5X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 3.1MP USB3.0 camera. The highly-sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. A reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/2.8"
    Active Pixels 3.1M (2048 x 1536)
    Pixel Size 2.5µm x 2.5µm
    Active Sensor Area 5.12mm x 3.84mm
    Shutter electronic rolling shutter
    Sensitivity 600mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 50fps @ 2048x1536
    Connectivity USB 3.0
    Lens-mount C-mount
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.5X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 6.3MP USB3.0 camera. The highly-sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. A 0.5X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/1.8"
    Active Pixels 6.3M (3072 x 2048)
    Pixel Size 2.4µm x 2.4µm
    Active Sensor Area 7.37mm x 4.92mm
    Shutter electronic rolling shutter
    Sensitivity 425mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 30 fps @ 3072x2048, 38fps @ 1536x1024
    Connectivity USB 3.0
    Lens-mount C-mount
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.5X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 12MP USB3.0 camera. The sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. A 0.75X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/1.7"
    Active Pixels 12M (4000 x 3000)
    Pixel Size 1.85µm x 1.85µm
    Active Sensor Area 7.4mm x 5.55mm
    Shutter electronic rolling shutter
    Sensitivity 280mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 25fps @ 4000x3000, 50fps @ 2048x1080
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.75X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.75X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included high-resolution 20MP USB3.0 camera. The sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 15X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1"
    Active Pixels 20M (5440 x 3648)
    Pixel Size 2.4µm x 2.4µm
    Active Sensor Area 13.06mm x 8.76mm
    Shutter electronic rolling shutter
    Sensitivity 462mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 15fps @ 5440x3648, 50fps @ 2736x1824, 60fps @ 1824x1216
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 1X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 20X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 1.2MP USB3.0 camera. With extraordinary sensitivity and high framerates, the MU123-FL provides high-definition imaging for low-light applications, including fluorescence and darkfield. Using a cutting-edge 1.2MP back-illuminated CMOS sensor with an incredible 2040mV sensitivity (1/30s), high gain-levels can be used for highly-responsive realtime video. A 0.37X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 20X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/3"
    Active Pixels 1.2M (1280 x 960)
    Pixel Size 3.75µm x 3.75µm
    Active Sensor Area 4.80mm x 3.60mm
    Shutter electronic rolling shutter
    Sensitivity 2040mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 60fps @ 1280x960
    120fps @ 640x480
    Connectivity USB 3.0
    Lens-mount C-mount
    Compensating Lens 0.37X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.37X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 2MP USB3.0 camera. With extraordinary sensitivity and a maximum framerate of 125fps, the MU203-BI provides high-definition imaging for low-light applications, including fluorescence and darkfield. Using a cutting-edge 2MP back-illuminated CMOS sensor with an incredible 2350mV sensitivity (1/30s), high gain-levels can be used for highly-responsive realtime video. A 0.37X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 20X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/2.8"
    Active Pixels 2M (1920 x 1080)
    Pixel Size 2.9µm x 2.9µm
    Active Sensor Area 5.57mm x 3.13mm
    Shutter electronic rolling shutter
    Sensitivity 1300mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 125fps @ 1920x1080
    Connectivity USB 3.0
    Lens-mount C-mount
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.5X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 3.1MP USB3.0 camera. The highly-sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. A reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 20X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format 1/2.8"
    Active Pixels 3.1M (2048 x 1536)
    Pixel Size 2.5µm x 2.5µm
    Active Sensor Area 5.12mm x 3.84mm
    Shutter electronic rolling shutter
    Sensitivity 600mV @ 1/30s
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 50fps @ 2048x1536
    Connectivity USB 3.0
    Lens-mount C-mount
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One 0.5X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

    The Viewing Head

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

    Optics

    The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The Stage

    The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

    Koehler Transmitted Illumination

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    Epi-fluorescence Illumination

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    Fluorescence

    Filters
    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm

    Digital Imaging

    Monitor and capture photos and videos with the included 6.3MP USB3.0 camera. The highly-sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. A 0.5X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Technical drawings with measurements (in mm)


    Optical System Infinity-corrected
    Reference Focal Length 220mm
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X, 20X
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Lenses Infinity-corrected
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Camera Specifications
    Sensor Type Back-illuminated CMOS
    Sensor Optical Format