AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-600X Magnification with 1.4MP Cooled Color CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-600X Magnification with 1.4MP Cooled Monochrome CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-600X Magnification with 6MP Cooled Color CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-900X Magnification with 1.4MP Cooled Color CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-900X Magnification with 1.4MP Cooled Monochrome CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-900X Magnification with 6MP Cooled Color CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-1200X Magnification with 1.4MP Cooled Color CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-1200X Magnification with 1.4MP Cooled Monochrome CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-1200X Magnification with 6MP Cooled Color CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-1500X Magnification with 1.4MP Cooled Color CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-1500X Magnification with 1.4MP Cooled Monochrome CCD Camera AmScope IN480 Series Inverted Epi-fluorescence Trinocular Compound Microscope 40X-1500X Magnification with 6MP Cooled Color CCD Camera

SKU: IN480T-FL-MF143C IN480T-FL-MF143 IN480T-FL-MF603 IN480TA-FL-MF143C IN480TA-FL-MF143 IN480TA-FL-MF603 IN480TB-FL-MF143C IN480TB-FL-MF143 IN480TB-FL-MF603 IN480TC-FL-MF143C IN480TC-FL-MF143 IN480TC-FL-MF603

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$10,886.99
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Key Features

  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative monochrome CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 600X magnification range using a quintuple lens turret for five unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 6MP highly-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative monochrome CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 900X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 6MP highly-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative monochrome CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 6MP highly-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 1.4MP ultra-sensative monochrome CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • 6MP highly-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
  • Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity1240mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate15fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (monochrome)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity2000mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate25fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format1"
 
Active Pixels6M (1360 x 1024)
 
Pixel Size4.54µm x 4.54µm
 
Active Sensor Area12.48mm x 9.99mm
 
Shutterglobal shutter
 
Sensitivity880mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate7.5fps @ 2748x2200
14fps @ 2748x1092
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens1X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 15X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity1240mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate15fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 15X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (monochrome)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity2000mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate25fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 15X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format1"
 
Active Pixels6M (1360 x 1024)
 
Pixel Size4.54µm x 4.54µm
 
Active Sensor Area12.48mm x 9.99mm
 
Shutterglobal shutter
 
Sensitivity880mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate7.5fps @ 2748x2200
14fps @ 2748x1092
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens1X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 20X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity1240mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate15fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shied
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 20X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (monochrome)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity2000mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate25fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 20X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format1"
 
Active Pixels6M (1360 x 1024)
 
Pixel Size4.54µm x 4.54µm
 
Active Sensor Area12.48mm x 9.99mm
 
Shutterglobal shutter
 
Sensitivity880mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate7.5fps @ 2748x2200
14fps @ 2748x1092
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens1X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 25X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity1240mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate15fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 25X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (monochrome)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity2000mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate25fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 25X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format1"
 
Active Pixels6M (1360 x 1024)
 
Pixel Size4.54µm x 4.54µm
 
Active Sensor Area12.48mm x 9.99mm
 
Shutterglobal shutter
 
Sensitivity880mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate7.5fps @ 2748x2200
14fps @ 2748x1092
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens1X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-600X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-600X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-600X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-900X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-900X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-900X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-1200X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-1200X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-1200X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-1500X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-1500X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound
Applications Clinic, Laboratory, Veterinary
Camera Compatibility PC & Mac
Camera Sensor Type CCD
Camera Type USB 3.0
Categories Microscopes, Compound Microscopes, Special Microscopes, Special Microscopes/Phase Contrast, Special Microscopes/EPI-Fluorescence, Special Microscopes/Inverted, Applications/Chemistry Microscopes, Applications/Medical & Microbiology Microscopes, Applications/Botany Microscopes/Phytopathology, Applications/Chemistry Microscopes/Biochemistry Microscopes, Applications/Chemistry Microscopes/Biotechnology Microscopes, Applications/Chemistry Microscopes/Pharmaceutics Microscopes, Applications/Medical & Microbiology Microscopes/Bacteriology, Applications/Medical & Microbiology Microscopes/Biochemistry, Applications/Medical & Microbiology Microscopes/Cell Culture, Applications/Medical & Microbiology Microscopes/Cytology, Applications/Medical & Microbiology Microscopes/Gout & Rheumatology, Applications/Medical & Microbiology Microscopes/Fluorescence, Applications/Medical & Microbiology Microscopes/Neuropathology, Applications/Medical & Microbiology Microscopes/Oncology, Applications/Medical & Microbiology Microscopes/Pathology, Applications/Medical & Microbiology Microscopes/Virology, Compound Microscopes/Brightfield, Student Microscopes/Graduate Student & Research Microscopes, Compound Microscopes/Phase Contrast, Compound Microscopes/EPI-Fluorescence, Compound Microscopes/Inverted
Condenser Type Brightfield, Phase Contrast
Education Level College, Teaching/Training
Eyepiece Mounting Size 30mm
Field of View Class Extreme Widefield
Head Style Trinocular
Illumination Bulb Count 1
Illumination Power 30W, 100W
Illumination Style Incident & Transmitted
Illumination Type Halogen, Fluorescent
Iris With Iris
Magnification 40X-1500X
Magnification Type Multi-Power
Objective Lens Power 4X, 10X, 20X, 40X, 60X
Objective Mounting Size 20mm
Optics Plan, Achromatic, Infinity
Power Compatibility Wide Band
product condition NEW
Product Type Category Compound

TPG

Enhance your microscopy experience by downloading the essential software for your microscope cameras. This software provides advanced features such as image capture, measurement tools, and editing capabilities, allowing you to maximize the potential of your microscope. Compatible with various camera models, it ensures seamless integration and optimal performance. Please use the software attached below to download the software and unlock the full capabilities of your microscope camera today.View our software licensing terms.

Operating System Software
Windows v.10.11.2024
MAC AmScopeAmLite_20240408.dmg
SDK TPG SDK (Optional) v.03.11.2024
Linux AmScopeAmLite_x64_x84_20231115.tar

AmScope products are warranted against manufacturer’s defects in material and workmanship from the original date of purchase, for the following years: 


• Five Year Warranty for Microscopes: All our microscopes come with a five (5) year factory warranty against manufacturing defects, covering labor and parts. 
• Ten Year Warranty for Euromex Microscopes: All our Euromex microscopes come with a ten (10) year factory warranty against manufacturing defects, covering labor and parts.
• One Year Warranty for Telescopes: All our telescopes come with a one (1) year warranty, except when specified in the description. 
• One Year Warranty for Accessories: All our microscope accessories (USB cameras, illuminators, illumination, dust covers, and etc.) come with a one (1) year warranty, except when specified in the description.
• One Year Warranty for Items labeled as “Product Ships From Manufacturer”: All products that are shipped directly from the manufacturer come with a one (1) year warranty, except when specified in the description. 

Warranty does not apply for consumable accessories and parts such as bulbs, batteries, fuses, cords, optical components or any add-on accessories such as mechanical specimen holders that are not built into the microscope stage as an integral part of the original manufacture, etc. Warranty does not cover microscopes, lenses cameras or other accessories that have become inoperable due to dirt or damage due to misuse or lack of maintenance. 

Note: Buyers are responsible for return shipping and handling cost for warranty services. Our warranty covers parts and labor only.

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity1240mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate15fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (monochrome)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity2000mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate25fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format1"
 
Active Pixels6M (1360 x 1024)
 
Pixel Size4.54µm x 4.54µm
 
Active Sensor Area12.48mm x 9.99mm
 
Shutterglobal shutter
 
Sensitivity880mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate7.5fps @ 2748x2200
14fps @ 2748x1092
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens1X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 15X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity1240mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate15fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 15X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (monochrome)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity2000mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate25fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 15X eyepieces expand the maximum magnification to 900X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 15X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format1"
 
Active Pixels6M (1360 x 1024)
 
Pixel Size4.54µm x 4.54µm
 
Active Sensor Area12.48mm x 9.99mm
 
Shutterglobal shutter
 
Sensitivity880mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate7.5fps @ 2748x2200
14fps @ 2748x1092
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens1X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 20X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity1240mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate15fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shied
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 20X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (monochrome)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity2000mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate25fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 20X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format1"
 
Active Pixels6M (1360 x 1024)
 
Pixel Size4.54µm x 4.54µm
 
Active Sensor Area12.48mm x 9.99mm
 
Shutterglobal shutter
 
Sensitivity880mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate7.5fps @ 2748x2200
14fps @ 2748x1092
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens1X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 25X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity1240mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate15fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 25X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (monochrome)
 
Sensor Optical Format2/3"
 
Active Pixels1.4M (1360 x 1024)
 
Pixel Size6.45µm x 6.45µm
 
Active Sensor Area8.77mm x 6.6mm
 
Shutterglobal shutter
 
Sensitivity2000mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate25fps @ 1360x1024
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens0.67X/0.75X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Mercury HBO Excitation
 Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
 B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
 G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm


 

Digital Imaging

The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

Technical drawings with measurements (in mm)


 

Optical SystemInfinity-corrected
 
Reference Focal Length220mm
 
HeadTrinocular, 45° incline
 
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
 
Ocular Diameter30mm
 
Ocular Dioptersleft ocular
 
Eyepieces10X, 25X
 
Photo-portCX-type height-adjustable, 23mm tube
 
Objective LensesInfinity-corrected
 
Objective Turretquintuple
 
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
 
Stagefixed-height, mechanical X-Y translation
 
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
 
Stage X-Y Travel Range120mm x 80mm
 
Specimen HoldersTerasaki plate, Petri dish
 
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
 
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
 
CondenserNA 0.3, centerable
 
Condenser Working Distance72mm maximum
 
Phase Contrast Annuli4X/10X and 20X/40X
 
Power90-240VAC 50/60Hz wide-band
 

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm


 

Camera Specifications
 
Sensor TypeInterline CCD (color)
 
Sensor Optical Format1"
 
Active Pixels6M (1360 x 1024)
 
Pixel Size4.54µm x 4.54µm
 
Active Sensor Area12.48mm x 9.99mm
 
Shutterglobal shutter
 
Sensitivity880mV @ 1/30s
 
Spectral Response380-650nm with IR-cut filter
 
Capture Resolution and Maximum Framerate7.5fps @ 2748x2200
14fps @ 2748x1092
 
ConnectivityUSB 3.0
 
Lens-mountC-mount
 
Compensating Lens1X
 

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


 

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shield
  • One C-mount TEC USB camera
  • One power adapter
  • One AC power cord (North America plug)
  • One USB 3.0 cable
  • One heavy-duty case