40X-1000X Upright Fluorescence Microscope with Rotating Multi-filter Turret + 12MP Color CCD Camera

SKU: FM820T-12CMC3

Availability: In Stock

Regular price $13,916.99 $10,019.99
>> How to Buy A Microscope

SELECTING A COMPOUND MICROSCOPE is as easy as 1-2-3...

Select the type of microscope you need or your industry

Select by type of microscope - Our Compound Microscope Assortment has been divided into major categories to make your shopping easy.

These categories include:

Brightfield, Darkfield, Phase-Contrast, Inverted and others

Choose a business application or industry to find the right compound microscope. Major industries or applicatins include:

Veterinary, Laboratory Use, Teaching, Metallurgical, Gemology and many others

Select a Magnification

AmScope offers an industry-leading selection of magnifications. All products are in-stock and usually ship the same day ordered

Select a Camera

We offer a wide variety of Cameras for every application - from student to professional use:

Select a type of camera - Eyepiece, USB 2.0 or USB 3.0 - to name the most popular choices

Choose the Megapixels of the camera - the higher the megapixel count on a camera sensor the greater the amount of detail that can be captured in an image

The Higher the Magnification - The More Detail You'll See

Magnification 40x
Magnification 1000x
Magnification 2500x

Magnification is the ability of your microscope to make small objects seem larger. As the magnification increases the area being viewed will be decrease is size but the detail of the area being viewed will improve.

Amscope microscopes come with multiple magnifications to aid you in finding the proper magnification for viewing each specimen.

Camera Connection Types

When comaring 2.0 and 3.0 there are few major differences. First the transfer rates. USB 2.0 offers transfer rates of 480 Mbps and USB 3.0 offers transfer rates of 4.8 Gbps - that's 10 times faster.

Camera Resolution

The most important factor in ensuring successful microscopic imaging is choosing the appropriate optics and camera for your application that gives you more data and higher image quality. When selecting a microscope camera, it is best to base your decision on the most important requirements for your microscopic observation.

Key Features

  • An infinity-corrected upright fluorescence microscope with a rotating 6-filter turret
  • Includes 4 excitation filter-blocks: ultraviolet, violet, blue, and green
  • Five magnification levels from 40X to 1000X
  • High-output 100W Mercury-vapor lamp
  • 12MP color CCD camera for low-light fluorescence applications

The FM820T is an upright fluorescence microscope with transmitted and reflected lighting, and a six-filter turret. The microscope is built around an infinity-corrected optical system with a 40X-1000X magnification range, and Koehler diascopic and episcopic illumination. The powerful 100W, wide-spectrum, mercury-vapor episcopic illuminator uses a rotary filter-selector which can house up to six fluorescence filter-blocks; already included are ultraviolet, violet, blue, and green excitation blocks. The sub-stage illuminator uses a 30W halogen lamp with field-lens and diaphragm for even, high-contrast lighting.

The backwards, quintuple nosepiece holds five plan-achromatic objective lenses, including: 4X, 10X, 20X, 40X, and 100X (oil). The Siedentopf-design head features a 30° incline, and a 48-75mm interpupillary-adjustment range. A trinocular photo-port provides a stable, dedicated mount for an imaging system. The microscope uses coaxial coarse and fine focus-adjustment, as well as coaxial control over X and Y translation of the 7.2" x 5.6" stage.

The 12MP color CCD digital camera and software suite provide a high-resolution imaging solution for fluorescence microscopy. The 1" format CCD sensor balances resolution and sensitivity with its 3.1µm pixels, favoring higher resolution while retaining moderate sensitivity. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Fluorescence

Filters
Filter Set Excitation Filter Dichromatic Mirror Emission Filter Common Stains*
UV-1A 330-385nm 400nm 420nm DAPI, Aniline Blue, BFP, BAO 9, DANSA, Hoechst (33258, 33342, 34580)
V 400-410nm 455nm 455nm
B 460-495nm 505nm 510-550nm Acridine Orange (DNA), Acridine Yellow, Alexa Fluor (430, 488, 500, 514),Cy2, DiO, FITC, Lucifer Yellow
G2 510-550nm 570nm 590nm Acridine Red, Alexa Fluor 532, NeuroTrace 530/615, Rhodamine B
*Common stains are provided as examples, and vary in emission strength. Stain specifications should be verified against filter and light-source specifications.
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. Some fluorophores may emit weak signals despite falling within filter specifications.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Specifications

Optical System Infinity-corrected
Head Trinocular, 30 ° incline, 360 ° rotatable
Interpupillary Adjustment Compensation-free gemel, 48-75mm
Eyepieces Plan 10X, high-eyepoint, FN 22mm, 30mm mount
Photo-port 23mm, compatible with reduction lenses
Nosepiece Quintuple reversed
Objective Lenses 4X, 10X, 20X, 40X (spring), 100X (spring, oil)
Focusing System Coaxial coarse and fine focus, upper limit-stop, tension-control
Stage Double-layer mechanical 185mm x 142mm
X-Y Translation Range 75mm x 55mm
Sub-stage Condenser-holder Rack-and-pinion, centerable
Condenser Achromatic swing condenser, NA0.9/0.25
Transmitted Illumination 30W halogen, continuous adjustment
Fluorescence Excitation Illumination 100W mercury-vapor HBO arc-lamp

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X Plan achromatic 0.10 28mm 0.17mm
10X Plan achromatic 0.25 10mm 0.17mm
20X Plan achromatic 0.40 5.1mm 0.17mm
40X Plan achromatic 0.65 0.7mm 0.17mm
100X Plan achromatic 1.25 0.18mm 0.17mm

Camera Specifications

Specifications

Sensor Specifications
Model Sony ICX834AQG (color)
Sensor Type interline CCD
Sensor Optical Format 1"
Active Pixels 12.1M (4284 x 2836)
Pixel Size 3.1µm x 3.1µm
Active Sensor Area 13.28mm x 8.79mm
Shutter global shutter
Sensitivity 420mV @ 1/30s (f/5.6)
Spectral Response 380-650nm with IR-cut filter
Other Camera Specifications
Binning 1x1, 2x2
Video Capture Framerate 3.6fps @ 4284x2836
3.6fps @ 2124x1418
Photo Capture Resolution 4284x2836
2124x1418
Connectivity USB 3.0
Lens-mount C-mount
Compensating Lens 1X (optional)
Power 5VDC over USB 3.0
Operating Temperature -10-50°C
Operating Humidity 30-80%

Software

OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

application Microscope-Clinic, Microscope-Veterinary, Microscope-Laboratory
camera compatibility PC & Mac
color Microscope-White
compatibility PC & Mac
field view Super widefield
head style Microscopes-Trinocular
illumination style Incident & Transmitted
light type Halogen, Fluorescent
magnification type Multi-Power
magnifying power Microscope-40X to 1000X
Manufacturer AmScope
microscope application Clinic, Veterinary, Laboratory, Teaching/Training
microscope camera pixel 12MP
microscope category Compound
microscope color White
microscope condenser design Abbe-Rack & Pinion w/ diaphram
microscope condenser type Brightfield, Kohler
microscope eyepiece power 10X
microscope eyepiece size 30mm
microscope field view Super Widefield
microscope head style Trinocular
microscope iris With Iris
microscope light power 30W, 100W
microscope light shape Epi, Built-in
microscope light type Halogen, Fluorescent
microscope magnifying power 40X to 1000X
microscope num of bulbs 2
microscope objective power 4X, 10X, 20X, 40X, 100X
microscope objective size 20mm
microscope optics Infinity, Achromatic, Plan
microscope power supply 110V/220V
microscope school College, Teaching/Training
microscope sensor CCD
microscope specialty EPI-Fluorescence
microscope stage Two-layer Mechanical Stage
microscope stage div 1.00mm
optics Infinity, Achromatic, Plan
pixel 12.0MP
Prop 65 Prop 65 Warning
specialty Microscope-EPI-Fluorescence

The FM820T is an upright fluorescence microscope with transmitted and reflected lighting, and a six-filter turret. The microscope is built around an infinity-corrected optical system with a 40X-1000X magnification range, and Koehler diascopic and episcopic illumination. The powerful 100W, wide-spectrum, mercury-vapor episcopic illuminator uses a rotary filter-selector which can house up to six fluorescence filter-blocks; already included are ultraviolet, violet, blue, and green excitation blocks. The sub-stage illuminator uses a 30W halogen lamp with field-lens and diaphragm for even, high-contrast lighting.

The backwards, quintuple nosepiece holds five plan-achromatic objective lenses, including: 4X, 10X, 20X, 40X, and 100X (oil). The Siedentopf-design head features a 30° incline, and a 48-75mm interpupillary-adjustment range. A trinocular photo-port provides a stable, dedicated mount for an imaging system. The microscope uses coaxial coarse and fine focus-adjustment, as well as coaxial control over X and Y translation of the 7.2" x 5.6" stage.

The 12MP color CCD digital camera and software suite provide a high-resolution imaging solution for fluorescence microscopy. The 1" format CCD sensor balances resolution and sensitivity with its 3.1µm pixels, favoring higher resolution while retaining moderate sensitivity. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Fluorescence

Filters
Filter Set Excitation Filter Dichromatic Mirror Emission Filter Common Stains*
UV-1A 330-385nm 400nm 420nm DAPI, Aniline Blue, BFP, BAO 9, DANSA, Hoechst (33258, 33342, 34580)
V 400-410nm 455nm 455nm
B 460-495nm 505nm 510-550nm Acridine Orange (DNA), Acridine Yellow, Alexa Fluor (430, 488, 500, 514),Cy2, DiO, FITC, Lucifer Yellow
G2 510-550nm 570nm 590nm Acridine Red, Alexa Fluor 532, NeuroTrace 530/615, Rhodamine B
*Common stains are provided as examples, and vary in emission strength. Stain specifications should be verified against filter and light-source specifications.
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. Some fluorophores may emit weak signals despite falling within filter specifications.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Specifications

Optical System Infinity-corrected
Head Trinocular, 30 ° incline, 360 ° rotatable
Interpupillary Adjustment Compensation-free gemel, 48-75mm
Eyepieces Plan 10X, high-eyepoint, FN 22mm, 30mm mount
Photo-port 23mm, compatible with reduction lenses
Nosepiece Quintuple reversed
Objective Lenses 4X, 10X, 20X, 40X (spring), 100X (spring, oil)
Focusing System Coaxial coarse and fine focus, upper limit-stop, tension-control
Stage Double-layer mechanical 185mm x 142mm
X-Y Translation Range 75mm x 55mm
Sub-stage Condenser-holder Rack-and-pinion, centerable
Condenser Achromatic swing condenser, NA0.9/0.25
Transmitted Illumination 30W halogen, continuous adjustment
Fluorescence Excitation Illumination 100W mercury-vapor HBO arc-lamp

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X Plan achromatic 0.10 28mm 0.17mm
10X Plan achromatic 0.25 10mm 0.17mm
20X Plan achromatic 0.40 5.1mm 0.17mm
40X Plan achromatic 0.65 0.7mm 0.17mm
100X Plan achromatic 1.25 0.18mm 0.17mm

Camera Specifications

Specifications

Sensor Specifications
Model Sony ICX834AQG (color)
Sensor Type interline CCD
Sensor Optical Format 1"
Active Pixels 12.1M (4284 x 2836)
Pixel Size 3.1µm x 3.1µm
Active Sensor Area 13.28mm x 8.79mm
Shutter global shutter
Sensitivity 420mV @ 1/30s (f/5.6)
Spectral Response 380-650nm with IR-cut filter
Other Camera Specifications
Binning 1x1, 2x2
Video Capture Framerate 3.6fps @ 4284x2836
3.6fps @ 2124x1418
Photo Capture Resolution 4284x2836
2124x1418
Connectivity USB 3.0
Lens-mount C-mount
Compensating Lens 1X (optional)
Power 5VDC over USB 3.0
Operating Temperature -10-50°C
Operating Humidity 30-80%

Software

OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

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